Equipment

  • 300kV cryo-TEM "Krios G4" with "Selectris X" energy filter, phase plate and Falcon4i

    300 kV cryogenic transmission electron microscope of the 4th generation Krios type with energy filter, phase plate and Falcon4i. The main areas of application are in structural biology and structural biochemistry as well as in chemistry.

    Suitable for cryogenic single particle analysis, tomography and MicroED.

    120 kV TEM "Talos" with cryostage

    120 kV transmission electron microscope of the Talos type equipped with a cryostage and suitable for tomography. The main fields of application are structural biology and structural biochemistry as well as chemistry.

    Suitable for room temperature single particle analysis (negative stain EM), cryogenic electron microscopy and tomography.

    30kV cryogenic FIB-SEM "Aquilos", correlative TEM

    For the production of ultrathin (<100 nm) lamellae for further use in cryogenic electron tomography or MicroED. The Aquilos is equipped with a correlative fluorescence microscope to localize potential specimens on the grid.

    Vitrobot Mark IV System "Plunger"

    Highly automated cryoEM grid preparation.

    Carbon coater

    Glow discharger/ Plasma cleaner

  • Yeast and bacterial cell culture

    Both organisms are mainly used for the expression of recombinant proteins, which we need for our structural biology work. Yeast is also an excellent eukaryotic model organism for genetic studies.

    Insect cell culture

    We use insect cells for the recombinant expression of soluble proteins and large protein complexes that we need for our structural biology work.

    Mammalian cell culture

    The mammalian cell culture is used to express the most difficult and largest protein complexes and membrane proteins, which do not give sufficient yield in other organisms.

    High performance liquid chromatographie (HPLC) - Kevin

    Our HPLC system Kevin with fraction collector is ideally suited for high-purity purification of the protein to be analyzed in a final step.

    Fast protein liquid chromatographie systems (FPLC) - Bob and Stuart

    We use our FPLC systems Bob and Stuart for batch purification of large amounts of protein. The systems are capable of performing affinity purification, size exclusion chromatography or both in succession.

    Platereader for fluorescence polarisation