- Renal development and function
Gene: GGT::Cre-ERT2
Dworniczak B, Skryabin B, Tchinda J, Heuck S, Seesing FJ, Metzger D, Chambon P, Horst J, Pennekamp P (2007) Inducible Cre/loxP recombination in the mouse proximal tubule. Nephron Experimental Nephrology 106: e11- 20. - Arterial hypertension with endothelial control
Gene: Conditional inactivation of the guanylyl cyclase-A gene in endothelial cells.
Sabrane K, Kruse MN, Fabritz L, Zetsche B, Mitko D, Skryabin BV, Zwiener M, Baba HA, M. Yanagisawa M, Kuhn M (2005) Vascular endothelium is critically involved in the hypotensive and hypovolemic actions of atrial natriuretic peptide. J Clin Invest 115: 1666-1674. - Cardiac hypertrophy
Construct: pMHCpolyA/hPP-I2
Kirchhefer U, Baba HA, Boknik P, Breeden KM, Mavila N, Bruchert N, Justus I, Matus M, Schmitz W, Depaoli-Roach AA, Neumann J (2005) Enhanced cardiac function in mice overexpressing protein phosphatase Inhibitor-2. Cardiovasc Res 68: 98-108. - Cardiac insufficiency
Construct: CREM IbDeltaC-X
Müller FU, Lewin G, Baba HA, Boknik P, Fabritz L, Kirchhefer U, Kirchhof P, Loser K,. Matus M, Neumann J, Riemann B, Schmitz W (2005) Heart-directed expression of a human cardiac isoform of cAMP-response element modulator in transgenic mice. J Biol Chem 280: 6906-6914. - Cardiac hypertrophy and cardiac insufficiency
Construct: paMHC-PP2A
Gergs U, Boknik P, Buchwalow I, Fabritz L, Matus M, IJustus I, Hanske G, Schmitz W, Neumann J (2004) Overexpression of the catalytic subunit of protein phosphatase 2A impairs cardiac function. J Biol Chem 279: 40827-34. - Skin sensitivity
Constructs: pAMM77-1 and pAMM69
Loser K, Mehling A, Apelt J, Stander S, Andres PG, Reinecker HC, Eing BR, Skryabin BV, G. Varga G, Schwarz T, Beissert S (2004) Enhanced contact hypersensitivity and antiviral immune responses in vivo by keratinocyte-targeted overexpression of IL-15. Eur J Immunol 34: 2022-2031. - Hypervolemic artherial hypertension
Construct: Global inactivation of the guanylyl cyclase-A gene.
Skryabin BV, Holtwick R, Fabritz L, Kruse MN, Veltrup I, Stypmann J, Kirchhof P, Sabrane K, Bubikat A, Voss M, Kuhn M (2004) Hypervolemic hypertension in mice with systemic inactivation of the (floxed) guanylyl cyclase-A gene by alphaMHC-Cre-mediated recombination. Genesis 39: 288-298. - Neuropsychiatric disease and behavioural changes
a) Gene: Deletion of the BC1 RNA gene
Lewejohann L, Skryabin BV, Sachser N, Prehn C, Heiduschka P, Thanos S, Jordan U, Dell'Omo G, Vyssotski AL, Pleskacheva MG, Lipp HP, Tiedge H, Brosius J, Prior H (2004) Role of a neuronal small non-messenger RNA: behavioural alterations in BC1 RNA-deleted mice. Behav Brain Res 154: 273-289.
Skryabin BV, Sukonina V, Jordan U, Lewejohann L, Sachser N, Muslimov I, Tiedge H, Brosius J (2003) Neuronal untranslated BC1 RNA: targeted gene elimination in mice. Mol Cell Biol 23: 6435-6441.
b) Gene: BC 200
Khanam T, Rozhdestvensky TS, Bundman M, Galiveti CR, Handel S, Sukonina V, Jordan U, Brosius J, Skryabin BV (2007) Two primate-specific small non-protein-coding RNAs in transgenic mice: neuronal expression, sub cellular localization and binding partners. Nucleic Acids Res 35: 529-539. - Cardiomyocytes hypertrophy
Gene: Conditional inactivation of the guanylyl cyclase-A gene in cardiomyocytes.
Holtwick R, van Eickels M, Skryabin BV, Baba HA, Bubikat A, Begrow F, Schneider MD, Garbers DL, Kuhn M (2003) Pressure-independent cardiac hypertrophy in mice with cardiomyocyte-restricted inactivation of the atrial natriuretic peptide receptor guanylyl cyclase-A. J Clin Invest 111: 1399-1407. - Role of S100A9 in inflammatory mechanisms
Gene: Deletion of the S100A9 gene.
Manitz MP, Horst B, Seeliger S, Strey A, Skryabin BV, Gunzer M, Frings W, Schonlau F, Roth J, Sorg C, Nacken W (2003) Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro. Mol Cell Biol 23: 1034-1043. - Arterial hypertension with smooth muscle control
Gene: Conditional inactivation of the guanylyl cyclase-A gene in smooth-muscle cells.
Holtwick R, Gotthardt M, Skryabin B, Steinmetz M, Potthast R, Zetsche B, Hammer RE, Herz J, Kuhn M (2002) Smooth muscle-selective deletion of guanylyl cyclase-A prevents the acute but not chronic effects of ANP on blood pressure. Proc Natl Acad Sci USA 99: 7142-7147. - Autosomal dominant polycystic kidney disease
Gene: Inactivation of the PKD-2 gene.
Pennekamp P, Karcher C, Fischer A, Schweickert A, Skryabin B, Horst J, Blum M, Dworniczak B (2002) The ion channel polycystin-2 is required for left-right axis determination in mice. Curr Biol 12: 938-943. - Model for the Prader Willi syndrome (PWS)
Gene: Deletion of cluster of the MBII-85 snoRNA genes.
Skryabin BV, Gubar LV, Seeger B, Pfeiffer J, Handel S, Robeck T, Karpova E, Rozhdestvensky TS, Brosius J (2007) Deletion of the MBII-85 snoRNA gene cluster in mice results in postnatal growth retardation. PLoS Genet 3: e235.