• Z/001/02 - Expression analysis of macrophage subpoulations during transmigration and diapedesis

    Prinicipal investigators: Johannes Roth and Clemens Sorg
    Participating institution: Institute of Experimental Dermatology
    Core Unit: Integrated Functional Genomics (IFG)
    Funding period: 06/2001 - 05/2004

    Technological / methodological innovation: Methods for systems biology / Affymetrix Gene Chip Technology

    Publication: Viemann D, Goebeler M, Schmid S, Klimmek K, Sorg C, Ludwig S, Roth J (2004) Transcriptional profiling of IKK2/NF-kappa B- and p38 MAP kinase-dependent gene expression in TNF-alpha-stimulated primary human endothelial cells. Blood 103 (9): 3365-3373. ABSTRACT

  • Z/002/07 - Development of a genomic oligonucleotide array for functional systems biology

    Prinicipal investigators: Carsten Müller-Tidow and Hubert Serve
    Participating institution: Department of Medicine A – Hematology and Oncology
    Core Unit: Integrated Functional Genomics (IFG)
    Funding period: 07/2006 - 12/2007

    Technological / methodological innovation:  Methods for systems biology / combination of chromatin immunoprecipitation (ChIP) with DNA microarray (chip) technology (ChIP-on-chip)

    Publication: Hoemme C, Peerzada A, Behre G, Wang Y, McClelland M, Nieselt K, Zschunke M, Disselhoff C, Agrawal S, Isken F, Tidow N, Berdel WE, Serve H, Müller-Tidow C (2008) Chromatin modifications induced by PML-RARalpha repress critical targets in leukemogenesis as analyzed by ChIP-Chip. Blood 111 (5): 2887-2895. ABSTRACT

  • Z/003/14 - Analysis of glycolipid receptors of microbial virulence factors in cell cultures and organs using imaging mass spectrometry

    MALDI-MS images acquired from coronal mouse brain slices. © American Chemical Society, 2014.

    Principal investigator: Klaus Dreisewerd
    Core Unit: None
    Funding period: 01/2014 - 03/2016

    Technological / methodological innovation: Increasing the sensitivity and resolution of the MALDI-MS imaging (MALDI-MSI for matrix-assisted laser desorption/ionization mass spectrometry imaging); the high analytical potential of the new methodology, named MALDI-2, was transferred into two new DFG-funded research projects, to further develop the technique. Additionally a prototype of a “coolMALDI” instrument was developed.

    Publications (selected):

    1. Pirkl A, Meier M, Popkova Y, Letzel M, Schnapp A, Schiller J, Dreisewerd K (2014) Analysis of free fatty acids by ultraviolet laser desorption ionization mass spectrometry using insect wings as hydrophobic sample substrates. Anal Chem 86: 10763-10771. ABSTRACT
    2. Soltwisch J, Kettling H, Vens-Cappell S, Wiegelmann M, Müthing J, Dreisewerd K (2015) Mass spectrometry imaging with laser-induced postionization. Science 348: 211-215. ABSTRACT
    3. Niehoff AC, Schulz J, Soltwisch J, Meyer S, Kettling H, Sperling M, Jeibmann A, Dreisewerd K, Francesconi KA, Schwerdtle T, Karst U (2016) Imaging by elemental and molecular mass spectrometry reveals the uptake of an arsenolipid in the brain of Drosophila melanogaster. Anal Chem 88: 5258-5263. ABSTRACT

    Patent pending:

    1. Bookmeyer C, Soltwisch J, Dreisewerd K (2020) Desorptions-Ionenquelle mit Dotiergas-unterstützter Ionisierung (Aktenzeichen: DE 10 2020 120 394.2, Anmeldedatum: 03.08.2020).
    2. Further nationalisations in China, USA and GB: Desorption Ion Source With Dopant-gas Assisted Ionization (CH 202110889226; US 17/392,592; GB 2110747).

  • Z/004/17 - Photoacoustic imaging (PAI) to non-invasively characterise atherosclerotic plaques in vivo

    Principal Investigators: Michael Schäfers, Sven Hermann
    Core Unit: Preclinical Imaging eXperts (PIX)
    Funding period: 1/2017 to 12/2019

    Technological / methodological innovation: Direct visualisation of inflammatory activity in atherosclerotic lesions. After tests with various photoacoustic imaging protocols, preliminary data indicate that absolute quantification in vivo cannot be achieved with state-of-the-art PA technology using dye-based tracers. Further experiments are being conducted.

    Publication: Roll W, Markwardt NA, Masthoff M, Helfen A, Claussen J, Eisenblätter M, Hasenbach A, Hermann S, Karlas A, Wildgruber M, Ntziachristos V, Schäfers M (2019) Multispectral optoacoustic tomography of benign and malignant thyroid disorders - a pilot study. J Nucl Med 60 (10): 1461-1466. ABSTRACT