The IZKF Münster is not solely a funding instrument in the context of the performance-oriented allocation of funds, but also hosts a technology platform within the Medical Faculty. Thus, part of the resources serve to improve the technologogy infrastructure. All researchers thus have access to state-of-the-art technologies for innovative research projects and can use the core units of the IZKF.
The former IZKF Core Unit TRAM was transferred to the Faculty of Medicine on 01.07.2015.
Core Facility TRAnsgeic animal and genetic engineering Models (TRAM)
Head: Dr. Dr. Boris Skryabin
The Bioinformatics and Genomics sections of the IZKF Core Unit Integrated Functional Genomics (IFG) IZKF were merged with the Core Facility of the LIFA on 01.10.2014 to form the new Core Facility Genomics of the Faculty of Medicine.
Head: Univ.-Prof Dr. Monika Stoll
Single cell analyses identify important new levels of information in cell-based research: in addition to understanding the cellular heterogeneity of tissues and the physiological development of organs, single cell transcriptome analyzes (scRNA-seq) provide a deep understanding of the development of diseases. Spatial transcriptomics (ST) provide important additional levels of information in addition to scRNA-seq: by integrating ST and scRNA-seq, for example, cellular heterogeneity can be correlated with histopathological properties of the tissue or the influence of cell types or anatomical niches on cell programs can be examined. ST combines transcriptional and anatomical information. The "CytAssist" enables standardised processing of ST samples through automated placement on ST-CHIPs. This standardisation of the work step results in multiple advantages compared to manual processing, such as i.) an exact and simple placement of the sample on the ST-CHIP; ii.) the use of samples already placed on a glass slide; iii.) Independence from specialised personnel. ST can be processed in very good quality on FFPE material, which makes ST an attractive technique in the field of single cell research of rare diseases.
The group of PD Dr. Kornelius Kerl / Clinic for Pediatric Hematology and Oncology was able to purchase the Visium CytAssist, 10x Genomics in December 2022 from rededicated IZKF funds. This device is available to all IZKF members. If you are interested in using the "CytAssist" or in single cell analyses in general, please contact PD Dr. Cornelius Kerl.
CONTACT PERSONS:
PD Dr. med. Kornelius Kerl
Clinic for Pediatric Hematology and Oncology
E-Mail: Kornelius Kerl
The IZKF Technology and Methods Service "Cardiovascular and Cellular Function (CarCeF)" is established at the Institute of Pharmacology and Toxicology. CarCeF provides a wide range of services as part of a comprehensive cardiovascular phenotyping of genetic mouse models in whole animals, in the isolated organ and at cellular level, but can also be applied to non-cardiovascular issues. CarCeF continues to provide the methods offered by the former IZKF Core Unit "CarTel". These methods are thus retained within the IZKF and the faculty will continue to have access to this field of expertise. Further information can be found in the Flyer
Laser capture microdissection (LCM) enables users to isolate small areas of tissue and even single cells from tissue sections under microscopic observation. These tiny isolated areas can then be further analyzed using analytical-chemical methods. A Zeiss PALM® CombiSystem (photo) previously located at the IZKF was recently moved to the Institute for Hygiene - Biomedical Mass Spectrometry, Robert-Koch-Straße 51, where it is available to all interested scientists of the Medical Faculty as an IZKF technology offer.
Contact:
Institute of Hygiene
- Biomedical Mass Spectrometry -
Julian Orthen
Tel.: +49 (0) 251 83-56272
Julian Orthen
Prof. Dr. Klaus Dreisewerd
Tel.: +49 (0) 251 83-56726
Klaus Dreisewerd
The IZKF technology platform provides an overview of the services offered by the core units. The brochure is updated at regular intervals. Innovations and enhancements to the individual core units are communicated via the brochure.
