• Regulation of organic cation transporters and interaction with intracellular proteins
• Role of  organic cation transporters in cellular toxicities of various drugs in rat, mouse and man
• Function of organic cation transporters in various diseases
• Analyses of acute rejection after kidney transplantation with modern imaging

Current techniques

• Isolation of single nephron segments of rat, mouse and man
• Generation of primary cultures of renal tubular cells of rat and mouse
• Culture of renal tubular cell lines of murine, porcine, canine, and human origin
• Transient and stable gene expression in HEK293-cells
• Generation and expression of mutants in HEK293-cells
• Split ubiquitin yeast two hybrid analysis of protein interactions
• Microfluorimetrical analysis of intracellular ion concentrations
• Microfluorimetrical analysis of organic cation transport via the fluorescent cation 4-di-1-ASP
• Quantitative and semi quantitative RT-PCR, and Western-blot analyses 
• Immuno-histochemistry, immuno-fluorescence
• Experimental kidney transplantation models in rats with and without acute and chronic rejection and immuno-suppression
• Ischemia/reperfusion models in rats and mice