Electron microscopy technology platform

The electron microscopy (EM) technology platform is located in the Institute for Musculoskeletal Medicine (IMM). Here we offer research groups scientific and technical support for their EM questions. We support projects starting with the choice of the right preparation techniques through to the scientific interpretation of the results obtained. The aim is the connection between ultrastructure and function to answer a large number of biological questions. The identification of subcellular structures serves to confirm and substantiate light microscopic and biochemical results and provides information that is difficult to obtain in any other way.

  • Team


    PD Dr. rer. nat. Uwe Hansen
    Head, Electron microscopy technology platform
    Room: 0.042
    Phone: +49 251 83-52970
    Fax: +49 251 83-57462
    E-Mail-Kontakt


    Karin Gäher
    Technical Assistant
    Room: 120.025
    Phone: +49-251-8352963
    Fax: +49-251-8357462
    E-Mail-Kontakt

  • Techniques

    We provide the following techniques:

    • Embedding in various synthetic resins
    • Production of semi and ultra thin sections
    • Transmission electron microscopy and immunogold electron microscopy
    • Freeze fracture replica techniques including immunogold labeling
    • surface replicas, e.g. to visualize the uptake of gold-marked substrates in cells
    • High-resolution platinum-carbon replicas e.g. from the cytoskeleton including immunogold mark
    • Negative contrasting and rotary evaporation of macromolecules including the isolation of authentic suprastructures with the help of paramagnetic Immunobeads

  • Publications

    Selected Publications

    1. Groh AC, Möller-Kerutt A, Gilhaus K, Höffken V, Nedvetsky P, Kleimann S, Behrens M, Ghosh S, Hansen U, Krahn MP, Ebnet K, Pavenstädt H, Ludwig A, Weide T. PALS1 is a key regulator of the lateral distribution of tight junction proteins in renal epithelial cells.J Cell Sci. 2024 Mar 1;137(5):jcs261303. doi: 10.1242/jcs.261303. Epub 2024 Jan 24. PMID: 38265145.
    2. Rotering H, Hansen U, Welp H and Dell´Aquilla AM (2020). Kaltes atmosphärisches Plasma und „advanced negative pressure wound therapy. Zeitschrift für Herz-, Thorax- und Gefäßchirurgie 34(1): 52-60.
    3. Chen Z, Kebschull L, Foll DA, Rauen U, Hansen U, Heitplatz B, Hessler M, Senninger N, Vogel T, Mohr A and Becker F. A (2020). Novel Histidine-Tryptophan-Ketoglutarate Formulation Ameliorates Intestinal Injury in a Cold Storage and ex vivo Warm Oxygenated Reperfusion Model in Rats. Biosci Rep 40, BSR20191989.
    4. Kronenberg D, Michel P, Hochstrat E, Wei M, Brinckmann J, Müller M, Frank A, Hansen U, Eckes B and Stange R (2020). Increased collagen turnover impairs tendon microstructure and stability in integrin α2β1 deficient mice. Int J Mol Sci 21 (8).
    5. Schwarz C, Hoerr V, Töre Y, Hösker V, Hansen U, Van De Vyver H, Niemann S, Kuhlmann MT, Jeibmann A, Wildgruber M and Faber C (2020). Isolating crucial steps in induction of infective endocarditis with preclinical modeling of host pathogen interaction. Frontiers in Microbiology 11, 1325.
    6. Stolberg-Stolberg J, Sambale M, Hansen U, Schäfer A, Raschke M, Bertrand J, Pap T and Sherwood J (2020). Cartilage trauma induces necroptotic chondrocyte death and expulsion of cellular contents. Int J Mol Sci 21, 4204.
    7. Chen Z, Mohr A, Heitplatz B, Hansen U, Pascher A, Brockmann JG and Becker F (2020). Sulforaphane Elicits Protective Effects in Intestinal Ischemia Reperfusion Injury. Int J Mol Sci 21(15).
    8. Hansen U, Holmes DF, Bruckner P and Bishop PN (2020). Analysis of opticin binding to collagen fibrils identifies a single binding site in the gap region and a high specificity towards thin heterotypic fibrils containing collagens II, and XI or V/XI. PLoS One 15(8):e0234672.
    9. Spenle C, Loustau T, Murdamoothoo D, Erne W, Beghelli-de la Forest Divonne S, Veber R, Petti L, Bourdely P, Morgelin M, Brauchle EM, Cremel G, Randrianarisoa V, Camara A, Rekima S, Schaub S, Nouhen K, Imhof T, Hansen U, Paul N, Carapito R, Pythoud N, Hirschler A, Carapito C, Dumortier H, Mueller CG, Koch M, Schenke-Layland K, Kon S, Sudaka A, Anjuere F, Van Obberghen-Schilling E and Orend G (2020). Tenascin-C orchestrates an immune suppressive tumor microenvironment in oral squamous cell carcinoma. Cancer Immunol Res 8(9): 1122-1138.
    10. Schmitz N, Timmen M, Kostka K, Hoerr V, Schwarz C, Faber C, Hansen U, Matthys R, Raschke MJ and Stange R (2020). A novel MRI compatible mouse fracture model to characterize and monitor bone regeneration and tissue composition. Scientific Reports 10(1):16238.
    11. Woltersdorf C, Bonk M, Leitinger B, Huhtala M, Käpylä J, Heino J, Gil Girol C, Niland S, Eble JA, Bruckner P, Dreier R, and Hansen U (2017). The binding capacity of α1β1-, α2β1- and α10β1-integrins depends on non-collagenous surface macromole­cules rather than the collagens in cartilage fibrils. Matrix Biology, in press.
    12. Van de Vyver, H, Bovenkamp PR, Hoerr V, Schwegmann K, Tuchscherr L, Niemann S, Kursawe L, Grosse C, Moter A, Hansen U, Neugebauer U, Kuhlmann MT, Peters G, Hermann S, and Löffler B. A novel mouse model of Staphylococcus aureus vas­cular graft infection: non-invasive imaging of biofilm development in vivo (2017). AJP 187 (2): 268-279.
    13. Gottardi R*, Hansen U*, Raiteri R, Loparic M, Düggelin M, Mathys D, Friederich NF, Bruck­ner P, and Stolz M (2016). Supramolecular organization of collagen fibrils in healthy and osteoarthritic human knee and hip joint cartilage. PLOS ONE 11 (10): e0163552.
    14. Zobel K, Hansen U, and Galla HJ (2016). Blood-brain barrier properties in vitro de­pend on composition and assembly of endogenous extracellular matrices. Cell Tissue Res 365 (2): 233-245.
    15. Linz A, Knieper Y, Gronau T, Hansen U, Aszodi A, Garbi N, Hammerling GJ, Pap T, Bruck­ner P, and Dreier R (2015). ER stress during the pubertal growth spurt results in im­paired long bone growth in chondrocyte-specific ERp57 knockout mice. J. Bone Mi­ner. Res. 30 (8): 1481-1493.
    16. Kalinka J, Hachmeister M, Geraci J, Sordelli D, Hansen U, Niemann S, Oetermann S, Pe­ters G, Löffler B, and Tuchscherr L (2014). Staphylococcus aureus isolates from chro­nic osteomyelitis are characterized by high host cell invasion and intracellular adapta­tion, but still induce inflammation. Int. J. Med. Microbiol. 304 (8): 1038-1049.
    17. Stange R, Kronenberg D, Timmen M, Peters M, Everding J, Hidding H, Eckes B, Han­sen U, Pap T, and Raschke MJ (2013). Age-related bone deterioration is dimin­ished by disrupted collagen sensing in integrin a2b1 deficient mice. Bone 56, 48-54.

     

User regulations

The usage and fee regualations can be found or downloaded here