In humans, less than 30% of natural and medically assisted conceptions result in live births. For successful pregnancy to occur, embryo adhesion to the endometrium is crucial and remains a limiting step in assisted reproduction. This process is critical during the window of implantation (WOI), when the endometrium is receptive to the blastocyst and involves trophectoderm cells attaching to the endometrial epithelium, leading to trophoblast outgrowth, endometrial tissue invasion, and placenta formation. Experimental evaluation of implantation is challenging and appropriate models to study the WOI functionally are lacking. The project will utilise a new 3D model of the endometrium to study the WOI. Culture conditions and hormonal treatments will be optimised to reflect endometrial receptivity phases, as verified by receptivity gene expression profiles and protein levels. Ultimately, our study aims to offer a novel tool to study the WOI in vitro and potentially predict pregnancy outcomes for clinicians.