Curriculum Vitae

2023-today PhD student at AG Brunotte, Institute of Molecular Virology, University Münster

                      CiM-IMPRS Graduate Program (associated member)

2021-2023 Research assistant at AG Voigt, Institute for Virology Essen, University hospital

                    Essen

2019-2021 Master of Science in Medical Biology, University Duisburg-Essen

2016-2019 Bachelor of Science in Bioscience/Life Science, University Münster

Project

While an infection with seasonal Influenza A virus (IAV) is accompanied by mild symptoms, and a balanced antiviral response, an infection with highly pathogenic Influenza of avian origin (HPAIV) can lead to a systemic infection and death caused by an imbalanced inflammation. However, the exact reasons behind the excessive host inflammatory response are still poorly understood.  Presumably Protein Kinase R (PKR) acts as a RIG-I independent viral RNA sensor in HPAIV, leading to the activation of a down streaming cascade involving p38 and MSK1 resulting in the phosphorylation of TRIM28. This then causes an increased production of proinflammatory cytokines. Since PKR acts as a central hub for detecting cellular stress and displays a lot of up- and downstream interaction partners it is critical to specify these interactions during infection to better understand PKRs role in excessive host inflammatory response. Therefore, the interactome of PKR will be investigated by using proximity labelling approach with the TurboID biotin ligase and Mass spectrometry over the course of IAV and HPAIV infections in lung epithelia cell lines.

Methods:

Cell culture, infection studies with IAV, HPAIV and SARS-CoV-2 (BSL-2, BSL-3), Western blot, quantitative RT-PCR, Plaque assay, cloning, inhibitor treatment, mass spectrometry, proteomics

Publications:

Malyshkina A, Bayer W, Podschwadt P, Otto L, Karakoese Z, Sutter K, Bruderek K, Wang B, Lavender KJ, Santiago ML, Leipe PM et al. Immunotherapy-induced cytotoxic T follicular helper cells reduce numbers of retrovirus-infected reservoir cells in B cell follicles. PLoS Pathog. 2023;19(10):e1011725. doi:10.1371/journal.ppat.1011725

Le-Trilling VTK, Banchenko S, Paydar D, Leipe PM et al. Structural mechanism of CRL4-instructed STAT2 degradation via a novel cytomegaloviral DCAF receptor. EMBO J. 2023;42(5):e112351. doi:10.15252/embj.2022112351

Awards:

Poster Award. 2022. Tag der Forschung of the medical faculty University Duisburg-Essen

Communication Award. 2022. Best “Three-minutes Thesis” Academy for Clinical and Experimental Virology (ACHIEVE) of the junge GfV