Publikationen

  • Publikations-Highlights

    Siebert-Kuss LM, Dietrich V, Di Persio S, Bhaskaran J, Stehling M, Cremers JF, Sandmann S, Varghese J, Kliesch S, Schlatt S, Vaquerizas JM, Neuhaus N, Laurentino S. (2024) Genome-wide DNA methylation changes in human spermatogenesis. Am J Hum Genet. 111(6):1125-1139. 

    Schülke LC, Wistuba J, Nordhoff V, Behre HM, Cremers JF, Kliesch S, Di Persio S, Neuhaus N. (2024) Identification of two hidden clinical subgroups among men with idiopathic cryptozoospermia. Hum Reprod. 39(5):892-901. 

    Di Persio S, Neuhaus N. (2023) Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing. Hum Reprod. 38(1):1-13.

    Sara Di Persio, Tobias Tekath, Lara Marie Siebert-Kuss, Jann-Frederik Cremers, Joachim Wistuba, Xiaolin Li, Gerd Meyer zu Hörste, Hannes C.A. Drexler, Margot Julia Wyrwoll, Frank Tüttelmann, Martin Dugas, Sabine Kliesch, Stefan Schlatt, Sandra Laurentino, Nina Neuhaus (2021) Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Reports Medicine. 2(9):100395.

  • Liste aller Publikationen

    • Stallmeyer B, Bühlmann C, Stakaitis R, Dicke AK, Ghieh F, Meier L, Zoch A, MacKenzie MacLeod D, Steingröver J, Okutman Ö, Fietz D, Pilatz A, Riera-Escamilla A, Xavier MJ, Ruckert C, Di Persio S, Neuhaus N, Gurbuz AS, Şalvarci A, Le May N, McEleny K, Friedrich C, van der Heijden G, Wyrwoll MJ, Kliesch S, Veltman JA, Krausz C, Viville S, Conrad DF, O'Carroll D, Tüttelmann F. (2024) Inherited defects of piRNA biogenesis cause transposon de-repression, impaired spermatogenesis, and human male infertility. Nat Commun. 15(1):6637.
    • Lahtinen AK, Funke M, Krallmann C, Wyrwoll MJ, Jarisch A, Yang Y, Bjarnason R, Romerius P, Sundin M, Norén-Nyström U, Langenskiöld C, Cremers JF, Kliesch S, Stukenborg JB, Neuhaus N, Jahnukainen K. (2024) Decreased spermatogonial numbers in boys with severe haematological diseases. Br J Haematol. 205(1):229-235.
    • Siebert-Kuss LM, Dietrich V, Di Persio S, Bhaskaran J, Stehling M, Cremers JF, Sandmann S, Varghese J, Kliesch S, Schlatt S, Vaquerizas JM, Neuhaus N, Laurentino S. (2024) Genome-wide DNA methylation changes in human spermatogenesis. Am J Hum Genet. 111(6):1125-1139.
    • Schülke LC, Wistuba J, Nordhoff V, Behre HM, Cremers JF, Kliesch S, Di Persio S, Neuhaus N. (2024) Identification of two hidden clinical subgroups among men with idiopathic cryptozoospermia. Hum Reprod. 39(5):892-901.
    • Sieper MH, Gaikwad AS, Fros M, Weber P, Di Persio S, Oud MS, Kliesch S, Neuhaus N, Stallmeyer B, Tüttelmann F, Wyrwoll MJ. (2024) Scrutinizing the human TEX genes in the context of human male infertility. Andrology. (3):570-584.
    • Duffin K, Neuhaus N, Andersen CY, Barraud-Lange V, Braye A, Eguizabal C, Feraille A, Ginsberg JP, Gook D, Goossens E, Jahnukainen K, Jayasinghe Y, Keros V, Kliesch S, Lane S, Mulder CL, Orwig KE, van Pelt AMM, Poirot C, Rimmer MP, Rives N, Sadri-Ardekani H, Safrai M, Schlatt S, Stukenborg JB, van de Wetering MD, Wyns C, Mitchell RT. (2024) A 20-year overview of fertility preservation in boys: new insights gained through a comprehensive international survey. Hum Reprod Open. 2024(2):hoae010.
    • Capponi C, Palazzoli M, Di Persio S, Fera S, Spadetta G, Franco G, Wistuba J, Schlatt S, Neuhaus N, de Rooij D, Vicini E. (2023) Interplay of spermatogonial subpopulations during initial stages of spermatogenesis in adult primates. Development. 150(10):dev201430.
    • Höffken V, Di Persio S, Laurentino S, Wyrwoll MJ, Terwort N, Hermann A, Röpke A, Oud MS, Wistuba J, Kliesch S, Pavenstädt HJ, Tüttelmann F, Neuhaus N, Kremerskothen J. (2023) WWC2 expression in the testis: Implications for spermatogenesis and male fertility. FASEB J.37(5):e22912.
    • Siebert-Kuss LM, Krenz H, Tekath T, Wöste M, Di Persio S, Terwort N, Wyrwoll MJ, Cremers JF, Wistuba J, Dugas M, Kliesch S, Schlatt S, Tüttelmann F, Gromoll J, Neuhaus N, Laurentino S. (2022) Transcriptome analyses in infertile men reveal germ cell-specific expression and splicing patterns. Life Sci Alliance. 6(2):e202201633.
    • Benninghoven-Frey KM, Neuhaus N, Lahtinen AK, Krallmann C, Portela JMD, Jarisch A, Nordhoff V, Soave A, Ba Omar HAM, Sundin M, Langenskiöld C, Kliesch S, Stukenborg JB, Jahnukainen K. (2022) Early testicular maturation is sensitive to depletion of spermatogonial pool in sickle cell disease. Haematologica. 107(4):975-979.
    • Schneider FJ, Dabel J, Wistuba J, Kliesch S, Schlatt S, Neuhaus N. (2022) New perspectives on fertility in transwomen with regard to spermatogonial stem cells. Reprod Fertil. RAF-22-0022.
    • Sara Di Persio,Tobias Tekath,Lara Marie Siebert-Kuss,Jann-Frederik Cremers,Joachim Wistuba,Xiaolin Li,Gerd Meyer zu Hörste,Hannes C.A. Drexler,Margot Julia Wyrwoll,Frank Tüttelmann,Martin Dugas,Sabine Kliesch,Stefan Schlatt,Sandra Laurentino, Nina Neuhaus (2021) Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Reports Medicine. 2(9):100395.
    • Funke M, Yang Y, Lahtinen A, Benninghoven-Frey K, Kliesch S, Neuheus N, Stukenborg JB, Jahnukainen K. (2021) Z-scores for comparative analyses of spermatogonial numbers troughout human development. Fertil Steril. 116(3):713-720.
    • Torres-Fernández LA, Emich J, Port Y, Mitschka S, Wöste M, Schneider S, Fietz D, Oud MS, Di Persio S, Neuheus N, Kliesch S, Hölzel M, Schorle H, Friedrich C, Tüttelmann F, Kolanus W. (2021) TRIM71 Deficiency Causes Germ Cell Loss During Mouse Embryogenesis and Is Associated With Human Male Infertility. Front Cell Dev Biol. 9:658966.
    • Schneider F, Dabel J, Sandhowe-Klaverkamp R, Neuheus N, Schlatt S, Kliesch S, Wistuba J. (2021) Serum and intratesticular inhibin B, AMH, and spermatogonial numbers in trans women at genderconfirming surgery: An observational study. Andrology. Online ahead pf print.
    • Di Persio S, Leitão E, Wöste M, Tekath T, Cremers JF, Dugas M, Li X, Zu Hörste GM, Kliesch S, Laurentino S, Neuheus N, Horsthemke B. (2021) Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes. Clin Epigenetics. 13(1):160.
    • Sara Di Persio,Tobias Tekath,Lara Marie Siebert-Kuss,Jann-Frederik Cremers,Joachim Wistuba,Xiaolin Li,Gerd Meyer zu Hörste,Hannes C.A. Drexler,Margot Julia Wyrwoll,Frank Tüttelmann,Martin Dugas,Sabine Kliesch,Stefan Schlatt,Sandra Laurentino, Nina Neuhaus (2021) Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis. Cell Reports Medicine. Online ahead of print.
    • Wyrwoll MJ, Temel ŞG, Nagirnaja L, Oud MS, Lopes AM, van der Heijden GW, Heald JS, Rotte N, Wistuba J, Wöste M, Ledig S, Krenz H, Smits RM, Carvalho F, Gonçalves J, Fietz D, Türkgenç B, Ergören MC, Çetinkaya M, Başar M, Kahraman S, McEleny K, Xavier MJ, Turner H, Pilatz A, Röpke A, Dugas M, Kliesch S, Neuhaus N; GEMINI Consortium, Aston KI, Conrad DF, Veltman JA, Friedrich C, Tüttelmann F. (2020) Bi-allelic mutations in M1AP are a frequent cause of meiotic arrest and severely impaired spermatogenesis leading to male infertility. Am J Hum Genet. 107(2):342-351.
    • Leitao E, Di Persio S, Laurentino S, Wöste M, Dugas M, Kliesch S, Neuhaus N, Horsthemke B. (2020) The sperm epigenome does not display recurrent epimutations in patients with severely impaired spermatogenesis. Clin Epigenetics 12(1):61.
    • Heckmann L, Langenstroth-Röwer D, Wistuba J, Portela JMD, Pelt AMM, Redmann K, Stukenborg JB, Schlatt S, Neuhaus N. (2020) The initial maturation status of marmoset testicular tissues has an impact on germ cell maintenance and somatic cell response in tissue fragment culture. Mol Hum Reprod. 26(6):374-388.
    • Portela JMD, Heckmann L, Wistuba J, Sansone A, Pelt AMMV, Kliesch S, Schlatt S, Neuhaus N. (2020) Development and Disease-Dependent Dynamics of Spermatogonial Subpopulations in Human Testicular Tissues. J Clin Med; 9(1).
    • Laurentino S, Heckmann L, Di Persio S, Li X, Meyer Zu Hörste G, Wistuba J, Cremers JF, Gromoll J, Kliesch S, Schlatt S, Neuhaus N. (2019) High-resolution analysis of germ cells from men with sex chromosomal aneuploidies reveals normal transcriptome but impaired imprinting. Clin Epigenetics; 11(1):127.
    • Sharma S, Schlatt S, Van Pelt A, Neuhaus N. (2019) Characterization and population dynamics of germ cells in adult macaque testicular cultures. PLoS One; 14(6):e0218194.
    • Heckmann L, Langenstroth-Röwer D, Pock T, Wistuba J, Stukenborg JB, Zitzmann M, Kliesch S, Schlatt S, Neuhaus N. (2018) A diagnostic germ cell score for immature testicular tissue at risk of germ cell loss. Hum Reprod. 33(4):636-645.
    • Tröndle I, Westernströer B, Wistuba J, Terwort N, Schlatt S, Neuhaus N. (2017) Irradiation affects germ and somatic cells in prepubertal monkey testis xenografts. Mol Hum Reprod 23(3):141-154.
    • Neuhaus N, Yoon J, Terwort N, Kliesch S, Seggewiss J, Huge A, Voss R, Schlatt S, Grindberg RV, Schöler HR. (2017) Single-cell gene expression analysis reveals diversity among human spermatogonia. Mol Hum Reprod 23(2):79-90.
    • Langenstroth-Röwer D, Gromoll J, Wistuba J, Tröndle I, Laurentino S, Schlatt S, Neuhaus N. (2017) De novo methylation in male germ cells of the common marmoset monkey occurs during postnatal development and is maintained in vitro. Epigenetics 12(7):527-539.
    • Neuhaus N. (2016) PRAME as diagnostic marker and as regulator for cell fate decisions in germ cell cancers. Br J Cancer 115(4):401-402.
    • Schneider F, Neuhaus N, Wistuba J, Zitzmann M, Heß J, Mahler D, van Ahlen H, Schlatt S, Kliesch S. (2015) Testicular Functions and Clinical Characterization of Patients with Gender Dysphoria (GD) Undergoing Sex Reassignment Surgery (SRS). J Sex Med. 12(11):2190-2200.
    • Schneider F, Redmann K, Wistuba J, Schlatt S, Kliesch S, Neuhaus N. (2015) Comparison of enzymatic digestion and mechanical dissociation of human testicular tissues. Fertil Steril 104:302-311.
    • Westernströer B, Langenstroth D, Kliesch S, Troppmann B, Redmann K, Macdonald J, Mitchell R, Wistuba J, Schlatt S, Neuhaus N. (2015) Developmental expression patterns of chemokines CXCL11, CXCL12 and their receptor CXCR7 in testes of common marmoset and human. Cell Tissue Res 361:885-98.
    • Westernströer B, Terwort N, Ehmcke J, Wistuba J, Schlatt S, Neuhaus N. (2015) Profiling of Cxcl12 receptors, Cxcr4 and Cxcr7 in murine testis development and a spermatogenic depletion model indicates a role for Cxcr7 in controlling Cxcl12 activity. PlosOne 9:e112598.
    • Langenstroth D, Kossack N, Westernströer B, Wistuba J, Behr R, Gromoll J, Schlatt S. (2014) Separation of somatic and germ cells is required to establish primate spermatogonial cultures. Hum Reprod 29: 2018-31.
    • Troppmann B, Kossack N, Nordhoff V, Schüring AN, Gromoll J. (2014) MicroRNA miR-513a-3p acts as a co-regulator of luteinizing hormone/chorionic gonadotropin receptor gene expression in human granulosa cells. Mol Cell Endocrinol. 390:65-72.
    • Kossack N, Terwort N, Wistuba J, Ehmcke J, Schlatt S, Schöler H, Kliesch S, Gromoll J. A combined approach facilitates the reliable detection of human spermatogonia in vitro. (2013) Hum Reprod. 28: 3012-25.
    • Eppelmann U, Gottardo F, Wistuba J, Ehmcke J, Kossack N, Westernstroeer B, Redmann K, Wuebbeling F, Burger M, Tuettelmann F, Kliesch S, Mallidis C. (2013) Raman microspectroscopic discrimination of TCam-2 cultures reveals the presence of two sub-populations of cells. Cell Tissue Res 354: 623-32.
    • Kossack N, Troppmann B, Richter-Unruh A, Kleinau G, Gromoll J. (2013) Aberrant transcription of the LHCGR gene caused by a mutation in exon 6A leads to Leydig cell hypoplasia type II. Mol Cell Endocrinol 366:59-67.
    • Albert S, Wistuba J, Eildermann K, Ehmcke J, Schlatt S, Gromoll J, Kossack N. (2012) Comparative marker analysis after isolation and culture of testicular cells from the immature marmoset. Cells Tissues Organs, 196:543-54.
    • Kossack N, Meneses J, Shefi S, Nguyen HN, Chavez S, Nicholas C, Gromoll J, Turek PJ, Reijo-Pera RA. (2009) Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells. Stem Cells 27:138-49.

  • Liste von Buchkapiteln und Reviews

    • Di Persio S, Neuhaus N. (2023) Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing. Hum Reprod. 38(1):1-13.
    • Goossens E, Jahnukainen K, Mitchell RT, van Pelt A, Pennings G, Rives N, Poels J, Wyns C, Lane S, Rodriguez-Wallberg KA, Rives A, Valli-Pulaski H, Steimer S, Kliesch S, Braye A, Andres MM, Medrano J, Ramos L, Kristensen SG, Andersen CY, Bjarnason R, Orwig KE, Neuhaus N, Stukenborg JB. (2020) Fertility preservation in boys: recent developments and new insights. Hum Reprod Open. hoaa016.
    • Neuhaus N, Schlatt S. (2019) Stem cell-based options to preserve male fertility. Science 363(6433):1283-1284.
    • Sharma S, Wistuba J, Pock T, Schlatt S, Neuhaus N. (2019) Spermatogonial stem cells: updates from specification to clinical relevance. Hum Reprod Update. 25(3):275-297.
    • Schneider F, Scheffer B, Dabel J, Heckmann L, Schlatt S, Kliesch S, Neuhaus N. (2019) Options for Fertility Treatments for Trans Women in Germany. J Clin Med;8(5).
    • Heckmann L, Pock T, Tröndle I, Neuhaus N. (2018) The C-X-C signalling system in the rodent vs primate testis: Impact on germ cell niche interaction. Reproduction. 155(5):R211-R219.
    • Westernströer B, Langenstroth D, Schlatt S, Kossack N. (2012) Repopulation of impaired seminiferous tubules. Stem cell based methods. Gynäkologische Endokrinologie 10: 245-249.
    • Kossack N, Chavez S, Reijo Pera RA. (2009) Germ cell-specific methylation pattern: Erasure and reestablishment, in C Simon and A Pellicer, Stem Cells in Human Reproduction (Basic Science and Therapeutic Potential, second edition), Informa Healthcare, New York, 43-57.
    • Kossack N, Gromoll J, Reijo Pera RA. (2009) Human embryonic stem cells and germ cell development, in VK Rajasekhar and MC Vemuri, Regulatory networks in stem cells, Humana Press, New York, 55-65.