Wechselwirkung zwischen pathogenen Escherichia coli (rot) und menschlichen Nierenzellen
© U. Dobrindt

    Genetische Grundlage der Bakterien-Wirt-Interaktion und Anpassung von Escherichia coli: Einfluss auf die Wechselwirkung an zellulären Barrieren

    Unser Ziel ist es, bakterielle Eigenschaften und die molekularen Mechanismen zu identifizieren, die zur unterschiedlichen Bakterien-Wirt-Interaktion im Rahmen einer Infektion oder Kolonisierung an zellulären Barrieren beitragen. Die Charakterisierung der bakteriellen Reduktion der RNA Polymerase II- und c-MYC-abhängigen Generegulation in Wirtszellen steht dabei im Vordergrund. Darüber hinaus untersuchen wir wie uropathogene E. coli durch epigenetische Mechanismen, wie Histonacetylierung und DNA-Methylierung, die angeborene Immunantwort während einer Infektion beeinflussen.

    Forschungsgebiet: Bakterielle Genetik, Infektionsbiologie

    Prof. Dr. rer. nat. Ulrich Dobrindt

    Projektlaufzeit: Juli 2012 - Juni 2024

    • Publikationen

      Originalartikel

      • Arvidsson I, Tontanahal A, Johansson K, Kristoffersson AC, Kellnerová S, Berger M, Dobrindt U, Karpman D (2022) Apyrase decreases phage induction and Shiga toxin release from E. coli O157:H7 and has a protective effect during infection. Gut Microbes 14(1): 2122667. doi: 10.1080/19490976.2022.2122667. 
      • Mukherjee K, Dobrindt U (2022) The emerging role of epigenetic mechanisms in insect defense against pathogens. Curr Opin Insect Sci. 49:8-14. doi: 10.1016/j.cois.2021.10.004.
      • Peng L, Dumevi RM, Chitto M, Haarmann N, Berger P, Koudelka G, Schmidt H, Mellmann A, Dobrindt U, Berger M (2022) A Robust One-Step Recombineering System for Enterohemorrhagic Escherichia coli. Microorganisms 10(9): 1689. doi: 10.3390/microorganisms10091689.
      • Ambite I, Filenko NA, Zaldastanishvili E, Butler DS, Tran TH, Chaudhuri A, Esmaeili P, Ahmadi S, Paul S, Wullt B, Putze J, Chen SL, Dobrindt U, Svanborg C (2021) Active bacterial modification of the host environment through RNA polymerase II inhibition. J Clin Invest. 131(4):e140333. doi:10.1172/JCI140333
      • Butler DSC, Cafaro C, Putze J, Wan MLY, Tran TH, Ambite I, Ahmadi S, Kjellström S, Welinder C, Chao SM, Dobrindt U, Svanborg C (2021) A bacterial protease depletes c-MYC and increases survival in mouse models of bladder and colon cancer. Nat Biotechnol 39(6): 754-764.  doi: 10.1038/s41587-020-00805-3. 
      • Gati NS, Temme IJ, Middendorf-Bauchart B, Kehl A, Dobrindt U, Mellmann A (2021) Comparative phenotypic characterization of hybrid Shiga toxin-producing / uropathogenic Escherichia coli, canonical uropathogenic and Shiga toxin-producing Escherichia coli. Int J Med Microbiol 311(7): 151533.  doi: 10.1016/j.ijmm.2021.151533. 
      • Bano L, Kiel M, Sales G, Doxey AC, Mansfield MJ, Wami HT, Schiavone M, Rossetto O, Pirazzini M, Dobrindt U, Montecucco C (2020) Genome Sequence of the Fish Brain Bacterium Clostridium tarantellae. Microbiol Resour Announc. 9(16):e01575-19. doi:10.1128/MRA.01575-19
      • Galstyan A, Ricker A, Nüsse H, Klingauf J, Dobrindt U (2020) Exploring the Impact of Coordination-Driven Self Assembly on the Antibacterial Activity of Low-Symmetry Phthalocyanines. ACS Appl Bio Mater. 3(1):400-411. doi:10.1021/acsabm.9b00873
      • Kalsy M, Tonk M, Hardt M, Dobrindt U, Zdybicka-Barabas A, Cytrynska M, Vilcinskas A, Mukherjee K (2020) The insect antimicrobial peptide cecropin A disrupts uropathogenic Escherichia coli biofilms. NPJ Biofilms Microbiomes. 6(1):6. doi:10.1038/s41522-020-0116-3
      • Leite Dantas R, Bettenworth D, Varga G, Weinhage T, Wami HT, Dobrindt U, Roth J, Vogl T, Ludwig S, Wixler V (2020) Spontaneous onset of TNFα-triggered colonic inflammation depends on functional T lymphocytes, S100A8/A9 alarmins, and MHC H-2 haplotype. J Pathol.251(4):388-399. doi:10.1002/path.5473
      • Mukherjee K, Amsel D, Kalsy M, Billion A, Dobrindt U, Vilcinskas A. (2020) MicroRNAs regulate innate immunity against uropathogenic and commensal-like Escherichia coli infections in the surrogate insect model Galleria mellonella. Sci Rep 13;10(1):2570. doi: 10.1038/s41598-020-59407-3. PMID: 32054914; PMCID: PMC7018962.
      • Gati NS, Middendorf-Bauchart B, Bletz S, Dobrindt U, Mellmann A (2019) Origin and Evolution of Hybrid Shiga Toxin-Producing and Uropathogenic Escherichia coli Strains of Sequence Type 141. Journal of clinical microbiology 58(1), e01309-19
      • Kalsy M, Tonk M, Hardt M, Dobrindt U, Zdybicka-Barabas A, Cytrynska M, Vilcinskas A, Mukherjee K (2020) The insect antimicrobial peptide cecropin A disrupts uropathogenic Escherichia coli biofilms. NPJ Biofilms Microbiomes 6(1):6
      • Leite Dantas R, Bettenworth D, Varga G, Weinhage T, Wami HT, Dobrindt U, Roth J, Vogl T, Ludwig S, Wixler V. (2020) Spontaneous onset of TNFα-triggered colonic inflammation depends on functional T lymphocytes, S100A8/A9 alarmins, and MHC H-2 haplotype. J Pathol 251(4):388-399
      • Mukherjee K, Amsel D, Kalsy M, Billion A, Dobrindt U, Vilcinskas A (2020) MicroRNAs regulate innate immunity against uropathogenic and commensal-like Escherichia coli infections in the surrogate insect model Galleria mellonella. Sci Rep 10(1):2570
      • Legros N, Ptaschek S, Pohlentz G, Karch H, Dobrindt U, Müthing J (2019) PapG Subtype-Specific Binding Characteristics of Escherichia coli Towards Globo-Series Glycosphingolipids of Human Kidney and Bladder Uroepithelial Cells. Glycobiology pii:cwz059.
      • Stork C, Kovács B, Rózsai B, Putze J, Kiel M, Dorn A, Kovács J, Melegh S, Leimbach A, Kovács T, Schneider G, Kerényi M, Emödy L, Dobrindt U (2018b) Characterization of asymptomatic E. coli bacteriuria isolates in search of alternative strains for efficient bacterial interference against uropathogens. Frontiers Microbiol 9:214.
      • Bielaszewska M, Rüter C, Bauwens A, Greune L, Jarosch AK, Steil D, Zhang W, He X, Lloubes R, Fruth A, Kim KS, Schmidt MA, Dobrindt U, Mellmann A, Karch H (2017) Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury. PLoS Pathogens 13:e1006159.
      • Galstyan A, Schiller R, Dobrindt U (2017b) Boronic Acid-Functionalized Photosensitizers: a Strategy to Target the Surface of Bacteria and Implement Active Agents in Polymer Coatings. Angew Chem Int Ed Engl 56:10362-10366.
      • Münzner P, Kengmo Tchoupa A, Klauser B, Putze J, Brunner T, Dobrindt U, Hauck CR (2016) Uropathogenic E. coli exploit CEA to promote colonization of the urogenital tract mucosa. PLoS Pathogens 12:e1005608.
      • Heitmueller M, Billion A, Dobrindt U, Vilcinskas A, Mukherjee K. (2017) Epigenetic Mechanisms Regulate Innate Immunity against Uropathogenic and Commensal-Like Escherichia coli in the Surrogate Insect Model Galleria mellonella. Infect Immun. 85(10). pii: e00336-17. (doi: 10.1128/IAI.00336-17)
      • Galstyan A, Putze J, Dobrindt U (2017) Striking an access to the bacteria via (reversible) control of lipophilicity.Chemistry. doi: 10.1002/chem.201704562.
      • Galstyan A, Schiller R, Dobrindt U. (2017) Boronic Acid Functionalized Photosensitizers: A Strategy to Target the Surface of Bacteria and Implement Active Agents in Polymer Coatings. Angew Chem Int Ed Engl. 56(35):10362-10366. (doi: 10.1002/anie.201703398)
      • Thölking G, Schuette-Nuetgen K, Vogl T, Dobrindt U, Kahl BC, Brand M, Pavenstädt H, Suwelack B, Koch R, Reuter S. (2017) Male kidney allograft recipients at risk for urinary tract infection? PLoS One 12(11):e0188262. doi: 10.1371/journal.pone.0188262.
      • Wiegand S, Zakrzewski SS, Eichner M, Schulz E, Günzel D, Pieper R, Rosenthal R, Barmeyer C, Bleich A, Dobrindt U, Schulzke JD, Bücker R. (2017) Zinc treatment is efficient against Escherichia coli α-haemolysin-induced intestinal leakage in mice. Sci Rep. 7:45649. (doi: 10.1038/srep45649)
      • Ambite I, Lutay N, Stork C, Dobrindt U, Wollt B, Svanborg C (2016) Bacterial suppression of RNA polymerase II-dependent host gene expression. Pathogens 5: 49. (doi:10.3390/pathogens5030049) 
      • Dobrindt U, Wullt B, Svanborg C (2016) Asymtomatic Bacteriuria as a Model to Study the Coevolution of Hosts and Bacteria. Pathogens 5:21.
      • Muenzner P, Kengmo Tchoupa A, Klauser B, Brunner T, Putze J, Dobrindt U, Hauck CR (2016) Uropathogenic E. coli exploit CEA to promote colonization of the urogenital tract mucosa. PLoS Pathog 12(5): e1005608. (doi:10.1371/journal. ppat.1005608) 
      • Starost LJ, Karassek S, Sano Y, Kanda T, Kim KS, Dobrindt U, Rüter C, Schmidt MA (2016) Pertussis Toxin Exploits Host Cell Signaling Pathways Induced by Meningitis-Causing E. coli K1-RS218 and Enhances Adherence of Monocytic THP-1 Cells to Human Cerebral Endothelial Cells. Toxins 8:E291.
      • Kunsmann L, Rüter C, Bauwens A, Greune L, Glüder M, Kemper B, Fruth A, Wai SN, He X, Lloubes R, Schmidt MA, Dobrindt U, Mellmann A, Karch H, Bielaszewska M (2015) Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain. Sci Rep, 5:13252.
      • Bielaszewska M, Schiller R, Lammers L, Bauwens A, Fruth A, Middendorf B, Schmidt MA, Tarr PIT, Dobrindt U, Karch H, Mellmann M (2014) Heteropathogenic virulence and phylogeny reveal phased pathogenic metamorphosis in Escherichia coli O2:H6. EMBO Mol Med 6(3): 347-357.
      • Köves B, Salvador E, Grönberg-Hernandez J, Zdziarski J, Wullt B, Svanborg C, Dobrindt U (2014) Rare emergence of symptoms during long-term asymptomatic E. coli 83972 carriage, without altered virulence factor repertoire. J Urol 191: 519-528. doi:10.1016/j.juro.2013.07.060.
      • Revelles O, Millard P, Nougayrède J-P, Dobrindt U, Oswald E, Létisse F, Portais J-C (2013) The Carbon storage regulator (Csr) system exerts a nutrient-specific control over central metabolism in Escherichia coli strain Nissle 1917. PLoS One 8: e66386
      • Sváb D, Horváth B, Maróti G, Dobrindt U, Tóth I (2013) Sequence variability of P2-like prophage genomes carrying the cytolethal distending toxin V operon in Escherichia coli O157. Appl Environ Microbiol 79: 4958-4964.
      Buchartikel
      • Rehm N, Berger M, Dobrindt U (2016) Bacterial Genome Plasticity. In: Encyclopedia of Evolutionary Biology (R.M. Kliman, ed.) Elsevier Amsterdam, in press.
      • Dobrindt U, Tjaden S, Shah S, Hacker J (2015b) Mobile genetic elements and pathogenicity islands encoding bacterial toxins. In: The Comprehensive Sourcebook of Bacterial Protein Toxins (Alouf, J., Ladant, D., Popoff, M. eds.) Elsevier Amsterdam, 40-76.